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additive screening

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(IUCr) Structure of the branched-chain keto acid decarboxylase ... Initial conditions were found using commercial crystallization screens; the small irregular crystals obtained from these could be optimized to single 0.1 × 0.1 × 0.2 mm crystals by additive screening. The optimized crystals were ...

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(IUCr) Purification, crystallization and data collection of the ... The initial crystal assumed the shape of a small diamond three months after crystallization setup with further optimization using additive screening (Fig. 1 [link] b). This type I crystal diffracted to 2.8 Å resolution (Fig. 2 [link] ...

(IUCr) Expression, crystallization and preliminary X-ray ... Additive screening was tried in order to improve the quality of crystals. For additive screening, drops were set up as follows: 2.5 µl protein solution plus 2.0 µl reservoir solution plus 0.5 µl additive screen (Hampton Research ...

(IUCr) Crystal optimization and preliminary diffraction data ... The crystals of Smad1 MH1-17-mer SBE with TT-AA overhang (SEB17-TTAA) showed initial weak diffraction to 10-17 Å resolution. We therefore selected this DNA element for further optimizations using additive screening (Hampton Research). ...

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Phys. Rev. B 78, 104111 (2008): Willot et al. - Effective-medium ... The zones where bands cross depicted in this figure either in the matrix zones c or close to the voids zones d + v are places of additive screening or enhancement of the stress. Thus, the parallel stress in zone c of map 20 reaches its ...

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(IUCr) Crystallization and preliminary X-ray diffraction analyses ... For optimization, additive screening kits from commercial screens (Hampton Research) were used in a high-throughput setting. Productive crystallization followed optimization by setting up droplets containing equal volumes of protein and ...

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(IUCr) Purification, crystallization and preliminary data analysis ... The diffraction of the blade-like crystals could be significantly improved by additive screening, with 30-50 mM Mg2+ as well as 5% DMSO shifting the diffraction limit to 2.0 Å (Fig. 1 [link] d). In contrast, the octahedron-like crystals ...

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(IUCr) Sugar-complex structures of the C-half domain of the ... Further additive screening was performed and the addition of cadmium ions improved the size and quality of the crystals. Finally, 5 µl protein solution containing 15 mg ml-1 GalNAc, 5 µl reservoir solution (0.2 M sodium chloride, ...

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(IUCr) Crystallization and preliminary X-ray analysis of the ... The crystallization conditions were improved using an additive screening kit (Hampton Research) and round-edged plate-shaped crystals were obtained from a droplet consisting of 1 µl 5 mg ml-1 protein solution and 1 µl reservoir solution ...

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(IUCr) Structure and biochemical characterization of an adenylate ... This condition was successively modified using streak-seeding with crushed crystals and additive screening, resulting in crystals that were suitable for data collection. The final crystallization condition was 1.5 M sodium citrate pH ...

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(IUCr) Surface-entropy reduction approaches to manipulate crystal ... More extensive additive screening identified 1% benzamidine-HCl as an additive that converted the initial needles into small blocky crystals. Subsequent optimization using microseeding techniques (5-12% PEG 3350, 0.1 M MES pH 6.5, ...

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grease testing equipment for a greener earth diesel fuel lubricity can be improved by the use of certain additives that work by increasing the efficiency of diesel fuel combustion, thereby reducing the consumption of fuels and the fuels costs incurred normally. ...

(IUCr) Expression, purification and preliminary X-ray analysis of ... Iterative rounds of detergent and additive screening improved the diffraction quality of the crystals as assessed by diffraction-based feedback using synchrotron radiation. Even using this procedure, the best crystals of crystal form 1 ...

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additive screening

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